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2010 Nanobiotechnology Seminar SeriesSeminar & Discussion 4:30 - 5:30 pm (otherwise noted below)Reception 5:30 - 6:00 pm (otherwise noted below) Seminars will be held in either the Clark Auditorium, Bio-X, Munzer Auditorium, Beckman Building or Center for Integrated Systems, CISX 101 Auditorium, Paul G. Allen Building Stanford University Campus You will need the free RealPlayer  to view the webcast of lectures and the Adobe Acrobat Reader plugin  to view the abstracts.
Current Seminar - 2010 Archived Nanobiotechnology Seminars: 2009 | 2008 | 2006-07 |
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| Jan 19, 2010 Munzer Auditorium  Edward H. Egelman, Ph.D. Professor Dept. of Biochemistry and Molecular Genetics University of Virginia View Webcast 
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Polymers and Pathogenesis: New Structural Insights
Abstract: Protein polymers are ubiquitous in biology, from cytoskeletal filaments to bacterial pili, and in many cases contain most of the protein in the cell. While it has been assumed that each polymer has a defined structure, we can show using electron cryo-microscopy and computational image analysis that many polymers exist in a multiplicity of states. Conserved subunits, such as bacterial flagellin or Type IV pilin, can be assembled in different ways, giving rise to abrupt changes in quaternary structure. As new quaternary structures emerge, these can have very new functions. For example, the bacterial ParM protein, a homolog of eukaryotic actin, forms filaments that are very different in structure than F-actin, and have very different functions. These insights suggest an under-appreciated mechanism for evolutionary divergence. |
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| Feb 16, 2010 Munzer Auditorium  Rashid Bashir, Ph.D. Bliss Professor Dept. of Electrical and Computer Engineering & Bioengineering University of Illinois |
Interfacing Biology and Silicon at the Micro and Nanoscale: Opportunities and Prospects
Abstract: Nanotechnology and BioMEMS can have a significant impact on medicine and biology in the areas of single cell detection, diagnosis and combating disease, providing specificity of drug delivery for therapy, and avoiding time consuming steps to provide faster results and solutions to the patient. Integration of biology and fabrication methods at the micro and nano scale offers tremendous opportunities for solving important problems in biology and medicine and to enable a wide range of applications in diagnostics, therapeutics, and tissue engineering. In this talk, we will present an overview of our work in Silicon-Based BioMEMS and Bionanotechnology and discuss the state of the art and the future challenges and opportunities. We will review a range of projects in our group focused towards developing rapid detection of biological entities and developing point of care devices using electrical or mechanical phenomenon at the micro and nano scale. We will present our work on developing silicon-based Petri dishes-on-a-chip, silicon based nano-pores for detection of DNA, silicon field-effect sensors for detection of DNA and proteins, and use of mechanical sensors for characterization of living cells. |
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| Mar 2, 2010 CISX 101 Auditorium  Mauro Ferrari, Ph.D. Professor Dept. of Experimental Therapeutics Univ. of Texas M.D. Anderson Cancer Center |
Designer Nanotherapeutics – A Prelude and Fugue
Abstract: TBA |
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| Apr 6, 2010 CISX 101 Auditorium  Alexander Wei, Ph.D. Professor of Chemistry and University Faculty Scholar Dept. of Chemistry Purdue University |
Targeted Delivery of Gold Nanorods and Other Plasmonic
Nanostructures: en route to Theragnosis
Abstract: Gold nanorods and nanostars can couple with electromagnetic irradiation at visible and near-infrared frequencies, and serve as multifunctional agents in biophotonic applications. These anisotropic nanostructures are capable of both linear and nonlinear optical responses, due in large part to polarization-sensitive modes that can be tuned by various structural and materials factors. Both types of particles have been used in biological imaging, but diverge with respect to their specific application. Gold nanorods are particularly efficient at converting optical energy into heat, and have been used to deliver intense photothermal effects with subcellular precision, guided by two-photon excited luminescence. Gold nanostars can be synthesized with magnetic cores to support a dynamic (gyromagnetic) mode of NIR imaging, effective at enhancing contrast in heterogeneous media such as those encountered in tissues. Recent advances will be presented in the context of their impact on theranostics and nanomedicine. |
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| Apr 20, 2010 CISX 101 Auditorium  Andreas Hoenger, Ph.D. Associate Professor Dept. of Molecular, Cellular and Developmental Biology University of Colorado at Boulder |
A multi-scale approach to cell structure and function
Abstract: TBA |
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| May 18, 2010 Clark Auditorium  Bruce Cohen, Ph.D. LBNL Staff Scientist Materials Sciences Division, The Molecular Foundry Biological Nanostructures Laboratory Lawrence Berkeley National Laboratory (LBNL) Smart Nanoparticles for Cellular Imaging
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Abstract: Certain nanoparticles possess unusual optical properties that may be of great value in imaging and microscopy. We have recently developed photoactivatable nanoparticles – called caged quantum dots – that are non-luminescent under typical microscopic illumination but can be activated with stronger pulses of UV light. These nanoparticles’ unique optical properties arise from the interaction between a classic organic caging group and a semiconducting quantum dot (QD), and while caging is dependent on the emission of the QD, it is effective through the visible spectrum into the nIR, offering a large array of new colors for photoactivatable probes. We have demonstrated that these QDs can be photoactivated within live cells and have examined the physical basis of the interaction between caging group and QD. For single molecule studies, we have found that a second type of nanoparticle – a lanthanide-doped upconverting nanoparticle (UCNP, below) – shows nearly ideal optical properties. UCNPs absorb two photons in the nIR and emit one at shorter wavelengths in the visible or nIR. UCNPs emit “anti-Stokes” light, producing a higher-energy photon from multiple lower-energy photos, and because nothing in the cell measurably emits anti-Stokes, there is minimal or no background autoflourescence. We have recorded the first single molecule images of UCNPs and find that they do not blink (as QDs ad many organic probes do) and that they posses remarkable photostability, resisting photobleaching under continuous irradiation long after organic dyes, proteins, and even QDs are extinguished. Through combinatorial methods, we have recently developed control over the both excitation and emission wavelengths of UCNPs, making multicolor upconverted imaging possible. | ||||
| June 22, 2010 Munzer Auditorium  Mansoor Amiji, Ph.D. Professor of Pharmaceutical Sciences Associate Chairman, Department of Pharmaceutical Sciences Co-Director, Nanomedicine Education and Research Consortium (NERC) Northeastern University |
Multifunctional Nanosystems for Early Diagnosis & Targeted Therapy
Abstract: There has been tremendous recent interest in nanotechnology application for disease prevention, diagnosis, and treatment. For many diseases, such as cancer, early diagnosis and overcoming biological barriers and target specific delivery are the key challenges. Additionally, newer generation of molecular therapies, such as gene therapy oligonucleotides, and RNA interference, require robust and highly specific intracellular delivery strategies for effective therapeutic outcomes. In this presentation, I will provide an overview of our work over few years in nanotechnology for target specific delivery of drugs and genes. We have developed metal, polymer, and lipid-based nano-platforms for diagnosis and delivery of therapeutics and image contrast agents. Peptide-modified gold nanostructures were developed for early cancer detection. Using biodegradable polymers, we have formulated nanocarriers for systemic delivery of hydrophobic anticancer drugs and therapeutic genes. Additionally, we have developed nanoemulsions, using oils rich in omega-3 polyunsaturated fatty acids, which can facilitate drug delivery across different biological barriers, such as the blood-brain barrier. |
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| July 20, 2010 Munzer Auditorium  Robert Blumenthal, Ph.D. Program Director Center for Cancer Reseach Nanobiology Program National Cancer Institute |
Nanochemistry in Membranes: Applications to Vaccines and Chemotherapy
Abstract: We are developing a new chemical nanobiology that involves reaction of photo-activable probes within a membrane, which serves as a 50 nm, highly organized hydrophobic container. We have used the membrane bilayer specific probe iodonaphthylazide (INA) that reacts with proteins and lipids following activation in situ either by direct UV irradiation or by energy transfer from a variety of donor chromophores. We have used this method in an analytic model to establish which proteins of the viral envelope penetrate the target cell membrane in the course of infection. The covalent modification of membrane proteins and lipids also modifies the function of membrane proteins. When applied to enveloped viruses, the treatment resulted in a complete loss of infectivity due to a loss of function of viral fusion proteins. We have shown the wide applicability of this inactivation technique to HIV, Influenza, Ebola, Marburg, Dengue and VEE viruses. By exclusively targeting the lipidic domain, exposed epitopes are preserved making the inactivated pathogens excellent vaccine candidates potentially applicable to cancer vaccines. When applied to whole cells the treatment resulted in loss of signaling function of cell surface receptors and loss of transport function of multi drug resistance transporters. Overall, photo-activation of INA in various cell lines, including those over-expressing the multi-drug resistance transporters leads to apoptosis. We are developing this new modality for cancer treatment using small hydrophobic molecules that can be turned into tumor killing toxic compounds by targeted radiation and ultrasound. I will also discuss ways in which light or heat can trigger physical-chemical changes in liposomal membranes resulting in localized release of drugs. |
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| Aug 17, 2010 Munzer Auditorium  Joe W. Gray, Ph.D. Staff Scientist/Direcor Life Sciences Division Dept. of Cancer and DNA Damage Responses Lawrence Berkeley National Laboratory |
An Omic View of Signaling in Cancer
Abstract: TBA |
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| Sept 21, 2010 CISX 101 Auditorium  Wah Chiu, Ph.D. Alvin Romansky Professor Depts. of Biochemistry and Molecular Biology, Molecular and Cellular Biology, Molecular Physiciology and Biophysics, Molecular Virology and Microbiology Baylor College of Medicine |
Visual Biology of Molecular Machines and Cells
Abstract: TBA |
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| Oct 19, 2010 CISX 101 Auditorium  Michael Heller, Ph.D. Professor Depts. of Electrical & Computer Engineering, Bioengineering, and Nanoengineering Univ. of California, San Diego |
Detection of cfc-DNA and other Cancer Related Nanoparticle Biomarkers Directly in Blood
Abstract: TBA |
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| Nov 16, 2010 CISX 101 Auditorium  Jan Schnitzer, M.D. President and Director Proteogenomics Research Institute for Systems Medicine (PRISM) |
Proteomic imaging of caveolae to pump nanoparticles into specific organs or tumors
Abstract: TBA |
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| Dec 14, 2010 CISX 101 Auditorium  Luke Lee, Ph.D. Lloyd Distinguished Professor Dept. of Bioengineering Director Biomolecular Nanotechnology Center Co-Director Berkeley Sensor & Actuator Center Univ. of California, Berkeley |
Satellite Nanoscopes for Living Cell Imaging and Gene Regulation
Abstract: TBA |
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Sponsored by: Center for Cancer Nanotechnology Excellence Focused on Therapy Response (CCNE) Program - NIH/NCI U54 (MIPS); Host: Director, Sanjiv Sam Gambhir, MD, PhD (sgambhir@stanford.edu) If you would like to be included on the CCNE email distribution list for weekly meeting reminders, contact Billie Robles. |
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| Updated January 5, 2010 | |||||
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