Dose% ResponseLOG Dose% Response2 10 0.30103 10 4 20 0.60205999 20 5 25 0.69897 25 8 40 0.90308999 40 10 50 1 50 20 70 1.30103 70 30 85 1.47712125 85 50 90 1.69897 90

## Now one can linearize this plot in order to obtain the dose at which 50% response occurs. This is done by doing a linear regression on the LOG dose vs. (Response-Median).

LOG Dose% Response% Response Median(% Response-Median)0.30103 10 45 -35 0.60205999 20 -25 0.69897 25 -20 0.90308999 40 -5 1 50 5 1.30103 70 25 1.47712125 85 40 1.69897 90 45

9:1