Determination of Vm and Km
Linear representation of the the Michaelis-Menten Equation
Lineweaver-Burke equationthis equation is written:
where is the change in plasma concentration at a given time interval. Sm is the average plasma concentration between two sampling times.
A plot of versus 1/Sm produces a straight line with slope Km/Vm and a y-intercept at 1/Vm.