Determination of Vm and Km

Linear representation of the the Michaelis-Menten Equation

Lineweaver-Burke equation
this equation is written:

where is the change in plasma concentration at a given time interval.  Sm is the average plasma concentration between two sampling times.

A plot of  versus 1/Sm produces a straight line with slope Km/Vm and a y-intercept at 1/Vm.