We provide technical support and individual or group training as needed for several software packages including both open Source/Free software packages and selected commercial software tools applicable for Molecular image analysis, quantification and visualization. Some of the most commonly used software tools in our facility are listed below along with brief summary of key features.
| Software |
Summary of key features |
AMIDE

Supported File Formats: Raw, DICOM, GIF, Acr/Nema, MicroPET data, ECAT, Interfile, Analyze, NIFTI
|
- Multi-data file loading and visualization
- Standard 2D orthognal (trans, coronal, saggital), Volume rendering
- ROI defintion and quantitation:
- 2D and/or 3D (Ellipsoid, Cylindrical, box, iso-contour and freehand), ROI Statistics outputs(mean, min, max, median, s.d) in units (%ID/g, SUV) of the underlying image pixel value
- Export capability (image and/or analysis outputs):
- ROI result to txt, image views to jpeg, image data to any supported file formats
- Other features:
- Co-registration using Fuducial Marker, Image fusion, Cropping, fly-through, line profile, math, filter and factor analysis.
|
GE Micro View

Supported File Formats: Raw, DICOM, JPEG, ECAT, Analyze, Vtk, HFH, MINC, TIFF, BMP, PNG, VFF and Pixmap format |
- Data loading and visualization
- Robust single data 2D viewing (trans, coronal, saggital), Volume rendering, Surface rendering (isosurface), Sub-volume viewing, Gray scale only
- ROI defintion and quantitation:
- ROIs (Box, cylinder), Image Statistics (Mean, max, s.d), Bone Measurements (Bone Mineral Density and Bone Volume Fraction (BVF)),Stereological parameters, Line measurement,
- Export capability (image and/or analysis outputs):
- uses export/save as from file menu to convert image data to different file format
- Other features:
- Zoom, Pan, Rotate, Line Profile, Histogram Plotting, Display window/Level control, Arbitrary oblique slicing, Image registration & fusion (commercial version available for Stanford users)
|
ImageJ

Supported File Formats: All standard file formats (jpeg, tiff, dicom, Fits, pgm avi, text images, avi, raw and image sequences) and more |
- Data loading and visualization
- Multiple data loading, Default 2D display with slice image scroll bar, Plugins available for 3D view (volume, surface rendering), Orthogonal view (axial, coronal, saggital)
- ROI defintion and quantitation:
- ROIs: Rectangular, elliptical, plogon, freehand, line, point, Measurement: roi statistics, area and volume, Histogram, Line profile, surface profile, Gel analysis tools, ROI manager tools
- Export capability (image and/or analysis outputs):
- Save As to export image data to different file format
- Other features
- Many other tools such as Cut, copy, past, crop, size, scale, rotate, Macros & plugins for extended features, Smoothing, sharpening, edge detection and median filtering
|
RT_image

Supported File Formats: DICOM complainant, Imports: MicroCT, MicroPET (Static & Dynamic), IVIS (2D/3D), BMP, JPG, TIF, Avi, interfile and Raw. |
- Data loading and visualization
- Multiple data loading, Orthogonal viewing of 3D volume, 3D MIP, Volume and surface rendering
- ROI defintion and quantitation:
- ROIs: freehand, Rec. elliptical, ellipsoid, intensity contour, region grow, line, ROI analysis: Grouping, Splitting & pixel statistics, histogram, MRI T1/T2 fitting, Dynamic PET time activity curve (TAC)
- Export capability (image and/or analysis outputs):
- Export image data to 2D/3D DICOM file format, image slice view and animations
- Other features
- Image Fusion, Filtering, Masking, registration, Normalization, Math, Re-sampling and ROI (cut, copy, & past capability
|
OsiriX

Supported File Formats: DICOM complian, Imports: TIFF(8,16,32 bits) JPEG, ANALYZE, PNG, JPEG, QuickTime, AVI, MPEG and Raw |
- Data loading and visualization
- Query/Retrieve from any PACS, Exclusive navigation and visualization of multimodality and multidimensional images, 2D, 3D, 4D and 5D viewers) , All advanced rendering modules: Multiplanar reconstruction (MPR), curved-MPR, Maximum Intensity Projection (MIP), Volume rendering, surface rendering
- ROI defintion and quantitation:
- ROIs: Polygons, Oval, Recg. Region grow, Length, Angle, Point, Grouping ROIs to form 3D ROIs, Pixel statistics, Polygon measurements, Volume measurement, Ejection fraction, T2 fit map and analysis using plug-in module
- Export capability (image and/or analysis outputs):
- Export file, Images views and movie capability
- Other features
- Image registration and fusion tools, Many Image processing tools (Convolution/Filter), VOI cutter, Plug-Ins architecture, advanced Image navigation and manipulation tools
|
| Software |
Summary of key features |
MicroPET ASI Pro VMTM

Supported File Formats: MicroPET images (Analyze file format only) MicroPET instrument software
|
- Data loading and visualization
- Multi-data loading (static or dynamic, Synchronized 2D Viewing (trans, coronal, saggital), Sine movie (over space or over time)
- ROI defintion and quantitation:
- ROIs: Ellipse, Rec and trace, Region grow (2D or 3D), Time Activity curve
(TAC), ROI measurement: Area, Volume, Min, Max, Mean, Median and Total, Line Profile.
- Export capability (image and/or analysis outputs):
- Save Volume (Analyze format), Save views to tiff
- Other features:
- Sum frames, Projections and transforms, Flip about Z, Smoothing tools, Clipping, Bed Sticher, Annotate, Registration and Fusion tool, and Compartment Modeling
|
Living Image

Supported File Formats: IVIS image data only (tif, txt), IVIS imaging system instrument software
|
- Data loading and visualization
- Multi-image data loading, Photographic and bioluminescent overlay image display, Image adjust, Correction/Filtering
- ROI defintion and quantitation:
- ROI: Circle, rectangle, auto, freehand, Measurement: ROI statistics of Count or Photon, Image information, Line profile, histogram
- Export capability (image and/or analysis outputs):
- Saves images as tif file format
- Other features:
- Planar image analysis, Image math, Spectral Unmixing, Point source fitting, 3D Fluorescence imaging tomography (FLIT), 3D Diffuse Luminescence tomography (DLIT)
|
Amira

Supported File Formats: TIFF (multichannel and multiframes), BMP, JPEG, PNG, SGI, Leica, Zeiss, BioRad, Olympus, MRC, DICOM, Analyze 3D, Nifti, HOC, SWC, FIDAP, I-DEAS, Fluent,Geometric modeling and CAD: DXF, STL, VRML, Inventor, CATIA 4/5, IGES
Full flexibility and extensibility |
- Data loading and visualization
- Orthogonal and oblique slicing, Volume rendering, Surface rendering, Isolines and isosurfaces, Multichannel imaging, Image fusion, Vector and tensor visualization,Structured network visualization, Support of structured / unstructured grids,Molecular simulation and visualization
- ROI defintion and quantitation:
- Volume, area, and distance measurements,Densitometry (gray value statistics),Filament network statistics,Co-localization analysis,Component separation and counting,Arithmetic operations on images, vector fields,and unstructured grids,Direct integration of the MATLAB® compute engine
- Export capability (image and/or analysis outputs):
- Save As to export image data to different file format
- Other features
- 2D and 3D image filtering,Surface generation, FEM grid generation,Interactive/automatic segmentation,Interactive/automatic slice alignment,Image registration and morphing,Tensor computation,Skeletonization and tracing of neural and vascular networks,Deconvolution and Z-drop correction,Dedicated editors for segmentation, tracing and filtering
|
CRi Maestro, Fluorescence Imaging

Supported File Formats: Cri Maestro image cube data, Maestro fluorescence imaging instrument software
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- Data loading and visualization
- Loading image cube,Auto and Manual unmix,Adjusting Brightness and Contrast Levels,Applying Overlays,Adjusting a Cube's RGB,Changing Components in a Composite Image,Advanced Display Controls
- Displaying the Intensity Legend
- ROI defintion and quantitation:
- Auto threshold segmentation region finding,Manual drawing, modifying and cloning region of interest,Defined region quantification
- Export capability (image and/or analysis outputs):
- Saving An Unmixed Result Set
- Other features
- Working With Saved Result Sets, Checking Spectral Library,Using Line Profiles to Analyze Signals,Comparing Multiple Images, Processing a Batch of image data
|
ART OptiView

Supported File Formats: TD file scan data of fluorescence |
- Data loading and visualization
- Multi-data file loading,Display of scan data images, data information, intensity map, Display windows for different data analysis tasks
- ROI defintion and quantitation:
- Intensity, lifetime and lifetime error maps,Measuremnt of distances,Perform selections and compute the average, standard deviation of ROIs
- Other features
- Depth, concentration and quality factor analysis,Topographic representation of depth superimposed on the specimen profile
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